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The Stem Cell Core at QBRI has expertise in generation of human and non-human primate induced pluripotent stem cells (iPSCs) and their karyotypic and pluripotency characterization and banking.
The core also provides genome engineering services to create isogenic human pluripotent stem cells (hPSCs) lines as well as gene knockout/in via CRISPR/Cas9, TALENs, Recombinases and transposases. Furthermore, retro and lenti viruses can be designed and packaged for various applications.
Assays for quality control
The following assays are offered for pluripotent stem cells and other cell types:
- Pluripotency FLOW analysis OR Immunocytochemistry
- Three germ layer differentiation Immunocytochemistry OR FLOW analysis
- Short Tandem Repeat (STR) Analysis
- G banding karyotyping
- Master and Working cell banks
Control iPSC lines
The core will generate control iPSC lines from dermal fibroblast and CD34+ hematopoietic stem cells from healthy Male and Female donor samples. These iPSC lines are fully characterized for pluripotency, Karyotyped and Banked. These lines will be available for researchers to use as control lines for their experiments.
The Core has expertise in directed differentiation of hPSCs into cortical neurons and currently establishing directed differentiation of pancreatic progenitor and insulin producing beta cells from hPSCs. Once established, the core will provide control (WT) neural precursor cells and pancreatic progenitor cells for directed differentiations into neurons and Beta cells respectively.
Training and Collaboration
Furthermore, the core is responsible for training and teaching of scientific staff in pluripotent stem cell maintenance and trouble shooting with directed differentiation protocols.
QBRI is currently looking to recruit highly-skilled individuals for a number of roles.
Inger van Steenoven, Nour K. Majbour, Nishant Vaikath, Henk W. Berendse, Wiesje M. van der Flier, Wilma D.J. van de Berg, Charlotte E. Teunissen, Afina W. Lemstra, and Omar M.A. El-Agnaf
Journal: Movement Disorders. Article DOI: 10.1002/mds.111, Article ID: MDS111, Internal Article ID: 15859369
Caroline Spasojevic, Elisabetta Marangoni, Sophie Vacher, Franck Assayag, Didier Meseure, Sophie Château-Joubert, Martine Humbert, Manale Karam, Jean Marc Ricort, Christian Auclair, Marie Regairaz and Ivan Bièche (2018).
Oncotarget. Doi: 10.18632/oncotarget.25292
Ali Abouelghar, Reem Hasnah, Ghina Taouk, Mohamad Saad and Manale Karam (2018).
In light of its mission towards capacity building within the field of Autism Spectrum Disorder (ASD) service provision, Qatar Biomedical Research Institute (QBRI) – a research institute within Hamad...
Qatar Biomedical Research Institute (QBRI), part of Hamad Bin Khalifa University (HBKU), today signed a memorandum of understanding (MoU) with the Shafallah Center, a non-profit center dedicated to ...
As part of its extensive, month-long Autism Awareness Campaign, QBRI participated in various events across Doha, to shed light on the importance of early detection of autism spectrum disorder (ASD) ...
Cerebrospinal and serum alpha-synuclein species as potential biomarkers for Parkinson’s disease